Jacobi Ulrike G, Akkers Robert C, Pierson Elisabeth S, Weeks Daniel L, Dagle John M, Veenstra Gert Jan C
Department of Molecular Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands.
EMBO J. 2007 Sep 5;26(17):3900-9. doi: 10.1038/sj.emboj.7601822. Epub 2007 Aug 16.
In addition to TATA-binding protein (TBP), a key factor for transcription initiation, the metazoan-specific TBP-like factor TLF/TRF2 and the vertebrate-specific factor TBP2/TRF3 are known to be required for transcription of specific subsets of genes. We have combined an antisense-knockdown approach with transcriptome profiling to determine the significance and biological role of TBP-independent transcription in early gastrula-stage Xenopus laevis embryos. Here, we report that, although each of the TBP family members is essential for embryonic development, relatively few genes depend on TBP in the embryo. Most of the transcripts that depend on TBP in the embryo are also expressed maternally and in adult stages, and show no functional specialization. In contrast, TLF is linked to preferential expression in embryos and shows functional specialization in catabolism. A requirement for TBP2 is linked to vertebrate-specific embryonic genes and ventral-specific expression. Therefore TBP paralogs are essential for the gene-regulatory repertoire that is directly linked to early embryogenesis.
除了转录起始的关键因子TATA结合蛋白（TBP）外，后生动物特有的TBP样因子TLF/TRF2和脊椎动物特有的因子TBP2/TRF3也已知是特定基因子集转录所必需的。我们将反义敲低方法与转录组分析相结合，以确定非洲爪蟾原肠胚早期胚胎中不依赖TBP的转录的意义和生物学作用。在此，我们报告，虽然TBP家族的每个成员对胚胎发育都是必不可少的，但胚胎中相对较少的基因依赖TBP。胚胎中依赖TBP的大多数转录本在母体和成年阶段也有表达，并且没有显示出功能特化。相反，TLF与胚胎中的优先表达相关，并在分解代谢中显示出功能特化。对TBP2的需求与脊椎动物特有的胚胎基因和腹侧特异性表达相关。因此，TBP旁系同源物对于直接与早期胚胎发生相关的基因调控库至关重要。
