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TBPL2/TFIIA 复合物通过卵母细胞特异性启动子的使用建立母体转录组。

TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage.

机构信息

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404, Illkirch, France.

Centre National de la Recherche Scientifique (CNRS), UMR7104, 67404, Illkirch, France.

出版信息

Nat Commun. 2020 Dec 22;11(1):6439. doi: 10.1038/s41467-020-20239-4.

DOI:10.1038/s41467-020-20239-4
PMID:33353944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7755920/
Abstract

During oocyte growth, transcription is required to create RNA and protein reserves to achieve maternal competence. During this period, the general transcription factor TATA binding protein (TBP) is replaced by its paralogue, TBPL2 (TBP2 or TRF3), which is essential for RNA polymerase II transcription. We show that in oocytes TBPL2 does not assemble into a canonical TFIID complex. Our transcript analyses demonstrate that TBPL2 mediates transcription of oocyte-expressed genes, including mRNA survey genes, as well as specific endogenous retroviral elements. Transcription start site (TSS) mapping indicates that TBPL2 has a strong preference for TATA-like motif in core promoters driving sharp TSS selection, in contrast with canonical TBP/TFIID-driven TATA-less promoters that have broader TSS architecture. Thus, we show a role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code.

摘要

在卵母细胞生长过程中，需要转录来创建 RNA 和蛋白质储备，以实现母体能力。在此期间，通用转录因子 TATA 结合蛋白 (TBP) 被其同源物 TBPL2（TBP2 或 TRF3）取代，后者对于 RNA 聚合酶 II 转录是必不可少的。我们表明，在卵母细胞中，TBPL2 不会组装成全型 TFIID 复合物。我们的转录分析表明，TBPL2 介导卵母细胞表达基因的转录，包括 mRNA 检测基因以及特定的内源性逆转录病毒元件。转录起始位点 (TSS) 作图表明，TBPL2 强烈偏爱核心启动子中的 TATA 样基序，从而导致尖锐的 TSS 选择，而与具有更广泛 TSS 结构的典型 TBP/TFIID 驱动的 TATA 缺失启动子形成对比。因此，我们通过使用特定的 TSS 识别密码，展示了 TBPL2/TFIIA 复合物在建立卵母细胞转录组中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/66ca6e3da56a/41467_2020_20239_Fig7_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/96a636cd3b21/41467_2020_20239_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/66ca6e3da56a/41467_2020_20239_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/ad7cbcde5d8b/41467_2020_20239_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/2e3a4e20e61d/41467_2020_20239_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/8868a5818829/41467_2020_20239_Fig3_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/dbfed846d2a3/41467_2020_20239_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e866/7755920/66ca6e3da56a/41467_2020_20239_Fig7_HTML.jpg

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