Sangameswaran B, Reddy T Chandraganth, Jayakar B
Vinayaka Mission's College of Pharmacy, Vinayaka Missions University, K.N. Patty, Salem-636 008, Tamil Nadu, India.
Phytother Res. 2008 Jan;22(1):124-6. doi: 10.1002/ptr.2250.
The present study was conducted to evaluate the hepatoprotective effect of Andrographis lineata (Acanthaceae) extracts in carbon tetrachloride-induced liver injury in rats. Male Wistar rats with chronic liver damage, induced by subcutaneous injection of 50% v/v carbon tetrachloride in liquid paraffin at a dose of 3 mL/kg on alternate days for a period of 4 weeks, were treated with methanol and aqueous extracts of A. lineata orally at a dose of 845 mg/kg/day. The biochemical parameters such as serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, serum bilirubin and alkaline phosphatase were estimated to assess the liver function. Histopathological studies of the liver were also carried out to confirm the biochemical changes. Histopathological examinations of liver tissue corroborated well with the biochemical changes. The activities of extracts were comparable to a standard drug. Hepatic steatosis, hydropic degeneration and necrosis were observed in the carbon tetrachloride treated group, while these were completely absent in the standard and extract treated groups. A. lineata extracts exhibited hepatoprotective action against carbon tetrachloride-induced liver injury. The present investigation established pharmacological evidence to support the folklore claim that it is used traditionally as a hepatoprotective agent.
本研究旨在评估线纹香茶菜（爵床科）提取物对四氯化碳诱导的大鼠肝损伤的保肝作用。雄性Wistar大鼠通过在液体石蜡中皮下注射50% v/v四氯化碳，剂量为3 mL/kg，隔天注射一次，持续4周，诱导慢性肝损伤，然后以845 mg/kg/天的剂量口服线纹香茶菜的甲醇提取物和水提取物进行治疗。通过检测血清谷氨酸草酰乙酸转氨酶、血清谷氨酸丙酮酸转氨酶、血清胆红素和碱性磷酸酶等生化参数来评估肝功能。还进行了肝脏组织病理学研究以证实生化变化。肝脏组织病理学检查与生化变化结果相符。提取物的活性与标准药物相当。在四氯化碳处理组中观察到肝脂肪变性、水样变性和坏死，而在标准药物组和提取物处理组中则完全没有这些情况。线纹香茶菜提取物对四氯化碳诱导的肝损伤具有保肝作用。本研究建立了药理学证据，以支持其传统上用作保肝剂的民间说法。
