石英晶体微天平表面的光衍生聚合物薄膜：用于碳水化合物 - 蛋白质相互作用的传感器
Photoderivatized polymer thin films at quartz crystal microbalance surfaces: sensors for carbohydrate-protein interactions.
作者信息
Pei Yuxin, Yu Hui, Pei Zhichao, Theurer Matthias, Ammer Carolin, André Sabine, Gabius Hans-Joachim, Yan Mingdi, Ramström Olof
机构信息
Department of Chemistry, KTH-Royal Institute of Technology, Teknikringen 30, S-10044 Stockholm, Sweden.
出版信息
Anal Chem. 2007 Sep 15;79(18):6897-902. doi: 10.1021/ac070740r. Epub 2007 Aug 18.
Abstract
Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo- or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.
摘要
基于全氟苯基叠氮的双连接策略开发了光衍生化聚合物包覆的金表面。镀金石英晶体微天平（QCM）晶体首先通过光或热解氮烯的形成和插入，用聚乙二醇（PEG）单层进行共价功能化。随后，聚合物表面用作碳水化合物衍生化光探针光插入的底物，产生用于选择性蛋白质结合的不同识别基序。所得坚固且生物相容的传感器表面应用于流通式QCM仪器，以实时监测凝集素与碳水化合物的相互作用。结果清楚地显示了预测的凝集素选择性，证明了该方法的适用性。
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