Ma Mingxiao, Jin Ningyi, Shen Guoshun, Zhu Guangze, Liu Hui Juan, Zheng Min, Lu Huijun, Huo Xiaowei, Jin Minglan, Yin Gefen, Ma Haili, Li Xu, Ji Yue, Jin Kuoshi
Genetic Engineering Laboratory, Academy of Military Medical Sciences, Changchun 130062, PR China.
Vet Immunol Immunopathol. 2008 Jan 15;121(1-2):1-7. doi: 10.1016/j.vetimm.2007.06.029. Epub 2007 Jun 28.
Two recombinant fowlpox viruses (rFPV-P1 and rFPV-IL18-2AP12A) containing foot-and-mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 were evaluated to determine their abilities to induce humoral and cellular responses in the presence or absence of swine IL-18 as genetic adjuvant. The ability to protect swine against homologous virus challenge was examined. All swine were given booster vaccinations at 21 days after the initial inoculation and were challenged 10 days after the booster vaccination. Control groups were inoculated with wild-type fowlpox virus (wtFPV). All animals vaccinated with rFPV-P12A and rFPV-IL18-P12A developed specific anti-FMDV ELISA antibody and neutralizing antibody and T-lymphocyte proliferation was observed. Cellular immune function was evaluated via examination of IFN-gamma production in swine peripheral blood serum. The results demonstrate the potential viability of a fowlpox virus-based recombinant vaccine in the control and prevention of FMDV infections.
对两种含有口蹄疫病毒（FMDV）衣壳多肽、O/NY00的3C编码区的重组禽痘病毒（rFPV-P1和rFPV-IL18-2AP12A）进行了评估，以确定它们在有或无猪白细胞介素-18作为基因佐剂的情况下诱导体液和细胞免疫反应的能力。检测了其保护猪免受同源病毒攻击的能力。所有猪在初次接种后21天进行加强免疫，并在加强免疫后10天进行攻毒。对照组接种野生型禽痘病毒（wtFPV）。所有接种rFPV-P12A和rFPV-IL18-P12A的动物均产生了特异性抗FMDV ELISA抗体和中和抗体，并观察到T淋巴细胞增殖。通过检测猪外周血血清中γ干扰素的产生来评估细胞免疫功能。结果证明了基于禽痘病毒的重组疫苗在控制和预防FMDV感染方面的潜在可行性。
