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编码口蹄疫病毒P12A和3C以及猪白细胞介素-18的质粒的免疫原性。

Immunogenicity of plasmids encoding P12A and 3C of FMDV and swine IL-18.

作者信息

Mingxiao Ma, Ningyi Jin, Juan Liu Hui, Min Zheng, Guoshun Shen, Guangze Zhu, Huijun Lu, Xiaowei Huo, Minglan Jin, Xu Li, Haili Ma, Yue Ji, Gefen Yin, Kuoshi Jin

机构信息

Genetic Engineering Laboratory, Academy of Military Medical Sciences, Changchun 130062, PR China.

出版信息

Antiviral Res. 2007 Oct;76(1):59-67. doi: 10.1016/j.antiviral.2007.05.003. Epub 2007 Jun 4.

DOI:10.1016/j.antiviral.2007.05.003
PMID:17606304
Abstract

In this paper, two recombinant plasmids (pVIR-P12AIL18-3C and pVIR-P12A-3C) containing foot and mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 and using/or not swine IL18 as a genetic adjuvant were constructed, and evaluated for their ability to induce humoral and cellular responses in mice and swine. In addition, the ability to protect swine against homologous virus challenge was examined. Mice and swine were given booster vaccination twice and once, respectively, and swine were challenged 10 days after the booster vaccination. Control groups were inoculated with pVAX1 or phosphate-buffered saline (PBS). All animals vaccinated with pVIR-P12AIL18-3C and pVIR-P12A-3C developed specific anti-FMDV ELISA antibody and neutralizing antibody and T lymphocyte proliferation and CTL cytotoxic activity was observed. In addition, we found that pVIR-P12AIL18-3C possessed stronger immunogenicity than pVIR-P12A-3C. The pVIR-P12AIL18-3C and pVIR-P12A-3C provided full protection in 3/4 and 2/4 swine from challenge with FMDV O/NY00, respectively. The results demonstrate the potential viability of a DNA vaccine in the control and prevention of FMDV infections.

摘要

本文构建了两种重组质粒（pVIR-P12AIL18-3C和pVIR-P12A-3C），其包含口蹄疫病毒（FMDV）衣壳多肽、O/NY00的3C编码区，并使用/或不使用猪IL18作为基因佐剂，评估了它们在小鼠和猪中诱导体液和细胞免疫反应的能力。此外，还检测了其保护猪抵抗同源病毒攻击的能力。小鼠和猪分别进行了两次和一次加强免疫，猪在加强免疫后10天受到攻击。对照组接种pVAX1或磷酸盐缓冲盐水（PBS）。所有接种pVIR-P12AIL18-3C和pVIR-P12A-3C的动物均产生了特异性抗FMDV ELISA抗体和中和抗体，并观察到T淋巴细胞增殖和CTL细胞毒性活性。此外，我们发现pVIR-P12AIL18-3C比pVIR-P12A-3C具有更强的免疫原性。pVIR-P12AIL18-3C和pVIR-P12A-3C分别为3/4和2/4的猪提供了抵抗FMDV O/NY00攻击的完全保护。结果证明了DNA疫苗在控制和预防FMDV感染方面的潜在可行性。

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