Ganesan P, Kumar Chandini S, Bhaskar N
Department of Meat, Fish and Poultry Technology, Central Food Technological Research Institute, Mysore 570 020, Karnataka, India.
Bioresour Technol. 2008 May;99(8):2717-23. doi: 10.1016/j.biortech.2007.07.005. Epub 2007 Aug 13.
In vitro antioxidant activities of three selected Indian red seaweeds - viz., Euchema kappaphycus, Gracilaria edulis and Acanthophora spicifera were evaluated. Total phenolic content and reducing power of crude methanol extract were determined. The antioxidant activities of total methanol extract and five different solvent fractions (viz., petroleum ether (PE), ethyl acetate (EA), dichloromethane (DCM), butanol (BuOH) and aqueous) were also evaluated. EA fraction of A. spicifera exhibited higher total antioxidant activity (32.01 mg ascorbic acid equivalent/g extract) among all the fractions. Higher phenolic content (16.26 mg gallic acid equivalent/g extract) was noticed in PE fraction of G. edulis. Reducing power of crude methanol extract increased with increasing concentration of the extract. Reducing power and hydroxyl radical scavenging activity of E. kappaphycus were higher compared to standard antioxidant (alpha-tocopherol). The total phenol content of all the seaweeds was significantly different (P<0.05). In vitro antioxidant activities of methanol extracts of all the three seaweeds exhibited dose dependency; and increased with increasing concentration of the extract.
对三种选定的印度红藻——即卡帕藻、可食江蓠和刺松藻进行了体外抗氧化活性评估。测定了粗甲醇提取物的总酚含量和还原能力。还评估了总甲醇提取物和五个不同溶剂馏分（即石油醚（PE）、乙酸乙酯（EA）、二氯甲烷（DCM）、丁醇（BuOH）和水相）的抗氧化活性。在所有馏分中，刺松藻的EA馏分表现出较高的总抗氧化活性（32.01毫克抗坏血酸当量/克提取物）。在可食江蓠的PE馏分中发现了较高的酚含量（16.26毫克没食子酸当量/克提取物）。粗甲醇提取物的还原能力随提取物浓度的增加而增强。与标准抗氧化剂（α-生育酚）相比，卡帕藻的还原能力和羟基自由基清除活性更高。所有海藻的总酚含量存在显著差异（P<0.05）。所有三种海藻甲醇提取物的体外抗氧化活性呈现剂量依赖性；并且随提取物浓度的增加而增强。
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