Helfre G, Farjanel J, Dubois P, Fonvieille J, Frey J
Biochim Biophys Acta. 1976 Mar 11;429(1):72-83. doi: 10.1016/0005-2744(76)90031-0.
Subcellular fractionation by differential centrifugation was used to study protocollagen proline hydroxylase (EC 1.14.11.2) localization from chick embryo liver. The fractions have been characterized by marker enzymes and electron microscopy. By these methods, it was observed that procollagen-proline-hydroxylase is concentrated in the microsomal fraction which is sedimented at 145 000 X g in 250 mM sucrose.
采用差速离心法进行亚细胞分级分离,以研究鸡胚肝脏中前胶原脯氨酸羟化酶(EC 1.14.11.2)的定位。通过标记酶和电子显微镜对各分级分离物进行了表征。通过这些方法观察到,前胶原脯氨酸羟化酶集中在微粒体分级分离物中,该分级分离物在250 mM蔗糖中于145000×g下沉淀。