迷迭香和鼠尾草(迷迭香叶和鼠尾草叶,唇形科)精油的抗菌和抗氧化特性。

Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils.

作者信息

Bozin Biljana, Mimica-Dukic Neda, Samojlik Isidora, Jovin Emilija

机构信息

Faculty of Medicine, Department of Pharmacy, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia.

出版信息

J Agric Food Chem. 2007 Sep 19;55(19):7879-85. doi: 10.1021/jf0715323. Epub 2007 Aug 21.

Abstract

The essential oils of rosemary ( Rosmarinus officinalis L.) and sage ( Salvia officinalis L.) were analyzed by means of gas chromatography-mass spectrometry and assayed for their antimicrobial and antioxidant activities. Antimicrobial activity was tested against 13 bacterial strains and 6 fungi, including Candida albicans and 5 dermatomycetes. The most important antibacterial activity of both essential oils was expressed on Escherichia coli, Salmonella typhi, S. enteritidis, and Shigella sonei. A significant rate of antifungal activity, especially of essential oil of rosemary, was also exhibited. Antioxidant activity was evaluated as a free radical scavenging capacity (RSC), together with the effect on lipid peroxidation (LP). RSC was assessed by measuring the scavenging activity of essential oils on 2,2-diphenyl-1-picrylhydrazil (DPPH) and hydroxyl radicals. Effects on LP were evaluated following the activities of essential oils in Fe(2+)/ascorbate and Fe(2+)/H2O2 systems of induction. Investigated essential oils reduced the DPPH radical formation (IC50 = 3.82 microg/mL for rosemary and 1.78 microg/mL for sage) in a dose-dependent manner. Strong inhibition of LP in both systems of induction was especially observed for the essential oil of rosemary.

摘要

采用气相色谱 - 质谱联用仪对迷迭香(Rosmarinus officinalis L.)和鼠尾草(Salvia officinalis L.)的精油进行了分析,并测定了它们的抗菌和抗氧化活性。针对13种细菌菌株和6种真菌(包括白色念珠菌和5种皮肤真菌)测试了抗菌活性。两种精油最重要的抗菌活性表现在大肠杆菌、伤寒沙门氏菌、肠炎沙门氏菌和宋内志贺氏菌上。还表现出显著的抗真菌活性,尤其是迷迭香精油。抗氧化活性通过自由基清除能力(RSC)以及对脂质过氧化(LP)的影响来评估。通过测量精油对2,2 - 二苯基 - 1 - 苦基肼(DPPH)和羟基自由基的清除活性来评估RSC。在Fe(2 +)/抗坏血酸和Fe(2 +)/H2O2诱导体系中,根据精油的活性评估其对LP的影响。所研究的精油以剂量依赖的方式减少了DPPH自由基的形成(迷迭香精油的IC50 = 3.82μg/mL,鼠尾草精油的IC50 = 1.78μg/mL)。尤其在两种诱导体系中,迷迭香精油对LP表现出强烈的抑制作用。

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