Universidade do Algarve, Faculdade de Ciências e Tecnologia, DQF, Edifício 8, Campus de Gambelas 8005-139 Faro, Portugal.
Nat Prod Res. 2011 Mar;25(5):526-41. doi: 10.1080/14786419.2010.499513.
Salvia officinalis L. oils were isolated from the plant's commercial dried aerial parts, by hydrodistillation, with different distillation times. The essential oils were analysed by gas chromatography and gas chromatography-mass spectrometry. The antioxidant ability was measured using a free radical scavenging activity assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH), a thiobarbituric acid reactive substances (TBARS) assay, a deoxyribose assay for the scavenging of hydroxyl radical, an assay for site-specific actions and a 5-lipoxygenase assay. Antibacterial activity was determined by the agar diffusion method. 1,8-Cineole, α-pinene and camphor were the dominant components of all the essential oils. The different hydrodistillation times did not affect the oil yield nor the relative amount of the oil components. The time of hydrodistillation influenced the antioxidant activity. With the DPPH method, the oils isolated for 2 and 3 h were stronger free radical scavengers, while with the TBARS method, the highest antioxidant values were obtained in the oils isolated for 30 min, 2 and 3 h. Hydroxyl radical scavenging and lipoxygenase activity assays showed the best results with oils isolated for 1 and 3 h. With the deoxyribose method, sage oils at concentrations <1000 mg L(-1) showed better activity than mannitol. The essential oil of S. officinalis showed very weak antimicrobial activity.
迷迭香精油从植物的商业干燥地上部分通过水蒸气蒸馏分离，不同的蒸馏时间。通过气相色谱和气相色谱-质谱分析精油。抗氧化能力通过使用 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性测定法、硫代巴比妥酸反应物质（TBARS）测定法、脱氧核糖测定法清除羟基自由基、位点特异性作用测定法和 5-脂氧合酶测定法来测量。通过琼脂扩散法测定抗菌活性。1,8-桉树脑、α-蒎烯和樟脑是所有精油的主要成分。不同的水蒸气蒸馏时间不影响油的产量或油成分的相对量。水蒸气蒸馏时间影响抗氧化活性。用 DPPH 法，蒸馏 2 和 3 小时分离的油是更强的自由基清除剂，而用 TBARS 法，在 30 分钟、2 小时和 3 小时分离的油中获得了最高的抗氧化值。羟基自由基清除和脂氧合酶活性测定显示，1 小时和 3 小时分离的油具有最佳效果。在脱氧核糖法中，浓度<1000mg/L 的迷迭香油比甘露醇具有更好的活性。迷迭香精油表现出非常弱的抗菌活性。
