Münzel Daniela, Lehle Karla, Haubner Frank, Schmid Christof, Birnbaum Dietrich E, Preuner Jürgen G
Department of Cardiothoracic Surgery, University Hospital Regensburg, D-93042 Regensburg, Germany.
Biochem Biophys Res Commun. 2007 Oct 19;362(2):238-44. doi: 10.1016/j.bbrc.2007.06.045. Epub 2007 Jun 18.
Diabetic endothelial dysfunction was characterized by altered levels of adhesion molecules and cytokines. Aim of our study was to evaluate the effects of diabetic serum on cell-growth and proinflammatory markers in human saphenous vein endothelial cells (HSVEC) from diabetic and non-diabetic patients. Diabetic serum showed (1) complementary proliferative activity for non-diabetic and diabetic HSVEC, (2) unchanged surface expression of adhesion molecules, and (3) elevated levels of sICAM-1 in HSVEC of all donors. The concentration of sVCAM-1 was increased only in diabetic cells. The proinflammatory state of diabetic HSVEC characterized by increased levels of cytokines was compensated. We concluded that even under normoglycemic conditions the serum itself contains critical factors leading to abnormal regulation of inflammation in diabetics. We introduced an in vitro model of diabetes representing the endothelial situation at the beginning of diabetes (non-diabetic cells/diabetic serum) as well as the diabetic chronic state (diabetic cells/diabetic serum).
糖尿病性内皮功能障碍的特征是黏附分子和细胞因子水平改变。我们研究的目的是评估糖尿病患者和非糖尿病患者的糖尿病血清对人隐静脉内皮细胞（HSVEC）的细胞生长和促炎标志物的影响。糖尿病血清显示：（1）对非糖尿病和糖尿病HSVEC具有互补的增殖活性；（2）黏附分子的表面表达未改变；（3）所有供体的HSVEC中可溶性细胞间黏附分子-1（sICAM-1）水平升高。可溶性血管细胞黏附分子-1（sVCAM-1）的浓度仅在糖尿病细胞中增加。以细胞因子水平升高为特征的糖尿病HSVEC的促炎状态得到了代偿。我们得出结论，即使在血糖正常的情况下，血清本身也含有导致糖尿病患者炎症调节异常的关键因素。我们引入了一种糖尿病体外模型，该模型代表糖尿病初期（非糖尿病细胞/糖尿病血清）以及糖尿病慢性状态（糖尿病细胞/糖尿病血清）下的内皮情况。
