Haubner Frank, Lehle Karla, Münzel Daniela, Schmid Christof, Birnbaum Dietrich E, Preuner Jürgen G
Department of Cardiothoracic Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, D-93042 Regensburg, Germany.
Biochem Biophys Res Commun. 2007 Aug 31;360(3):560-5. doi: 10.1016/j.bbrc.2007.06.044. Epub 2007 Jun 18.
Hyperglycemia is the major cause of diabetic angiopathy. Aim of our study was to evaluate the impact of high glucose on cell growth and function of human "diabetic" endothelial cells (EC). Incubation of non-diabetic EC with glucose moderately inhibited cell growth and increased the expression of ICAM-1 and E-selectin. In the disease-specific EC, glucose treatment resulted also in moderately inhibited cell growth by 5-10%, increased basal expression of VCAM-1 by 10-20%, and an enhanced release of monocyte-chemoattractant-protein-1 (MCP-1) by 40-70%. The expression of ICAM-1 and E-selectin and the release of IL-6 and IL-8 was not affected. The usage of our disease-specific EC model might evaluate the impact of systemic factors of diabetic patients in the progression of endothelial dysfunction, and may be suitable to develop relevant therapeutic regimens.
高血糖是糖尿病血管病变的主要原因。我们研究的目的是评估高糖对人“糖尿病”内皮细胞（EC）细胞生长和功能的影响。用葡萄糖孵育非糖尿病EC适度抑制细胞生长，并增加细胞间黏附分子-1（ICAM-1）和E-选择素的表达。在疾病特异性EC中，葡萄糖处理也导致细胞生长适度抑制5%-10%，血管细胞黏附分子-1（VCAM-1）的基础表达增加10%-20%，单核细胞趋化蛋白-1（MCP-1）的释放增加40%-70%。ICAM-1和E-选择素的表达以及白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的释放不受影响。我们的疾病特异性EC模型的应用可能评估糖尿病患者全身因素对内皮功能障碍进展的影响，并且可能适合于制定相关治疗方案。
