临床标本中观察到的巨细胞病毒DNA聚合酶Ⅲ区突变所导致的生长和耐药表型。
Growth and drug resistance phenotypes resulting from cytomegalovirus DNA polymerase region III mutations observed in clinical specimens.
作者信息
Chou Sunwen, Marousek Gail I, Van Wechel Laura C, Li Shaobing, Weinberg Adriana
机构信息
Divisionof Infectious Disease, Oregon Health Science University, VA Medical Center, Portland, OR 97239, USA.
出版信息
Antimicrob Agents Chemother. 2007 Nov;51(11):4160-2. doi: 10.1128/AAC.00736-07. Epub 2007 Aug 20.
Abstract
Recombinant phenotyping of cytomegalovirus (CMV) pol region III mutations from clinical specimens showed that T813S and G841A each conferred foscarnet resistance and approximately threefold increased ganciclovir resistance; adding the UL97 mutation C592G increased ganciclovir resistance to approximately sixfold. Bacterial artificial chromosome CMV clones containing pol mutation L845P were nonviable unless repaired with the wild-type sequence.
摘要
对临床样本中巨细胞病毒（CMV）聚合酶（pol）区域III突变进行的重组表型分析表明，T813S和G841A各自赋予了膦甲酸钠抗性，并使更昔洛韦抗性增加了约三倍；添加UL97突变C592G使更昔洛韦抗性增加至约六倍。含有pol突变L845P的细菌人工染色体CMV克隆无法存活，除非用野生型序列进行修复。
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