Dias Fernando Braga Stehling, Diotaiuti Liléia, Romanha Alvaro José, Bezerra Cláudia Mendonça, Machado Evandro Marques de Menezes
Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisas René Rachou-Fiocruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil.
Mem Inst Oswaldo Cruz. 2007 Aug;102(5):643-5. doi: 10.1590/s0074-02762007005000057.
The aim of this work was to identify and report the occurrence of Trypanosoma rangeli and Trypanosoma cruzi in naturally infected Rhodnius nasutus (Hemiptera, Reduviidae, Triatominae) in the state of Ceará, Brazil. Triatomines feces, salivary glands, and hemolymph were collected for fresh examination, and specific detection of T. rangeli and T. cruzi DNA by polymerase chain reaction was carried out. The specific characterization of these two parasites showed the simultaneous presence of both parasites in two (7.7%) of the 26 positive insects. Our results provide further knowledge on the geographical distribution of T. rangeli in Brazil.
这项工作的目的是识别并报告在巴西塞阿拉州自然感染的鼻红猎蝽（半翅目，猎蝽科，锥蝽亚科）中克氏锥虫和兰氏锥虫的发生情况。收集锥蝽的粪便、唾液腺和血淋巴进行新鲜样本检查，并通过聚合酶链反应对兰氏锥虫和克氏锥虫的DNA进行特异性检测。对这两种寄生虫的特异性特征分析表明，在26只阳性昆虫中有两只（7.7%）同时存在这两种寄生虫。我们的结果为巴西兰氏锥虫的地理分布提供了更多信息。
