Wilson Rab, Monaghan Paul, Bowden Stephen A, Parnell John, Cooper Jonathan M
Department of Electronics and Electrical Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, G12 8LT, UK.
Anal Chem. 2007 Sep 15;79(18):7036-41. doi: 10.1021/ac070994c. Epub 2007 Aug 21.
A simple surface-enhanced Raman spectroscopy (SERS) microflow cell was developed to investigate distributions of scytonemin pigment within cyanobacteria from samples of rock collected from an arctic desert that contained endolithic cyanobacteria. The assay, which has future potential use in a variety of applications, including astrobiology and analysis of microorganisms in remote environments, involved studying SERS spectra of bacteria from within geological samples. By using a dispersed colloidal substrate in the microfluidic device, surface enhancement of the order >10(5) was obtained for the determination of the pigment in the microorganisms when compared to the native Raman spectra. The SERS assay, which had a nM sensitivity for scytonemin, showed that the concentration of pigment was highest in samples that had experienced the highest stress environments, as a result of high doses of UV irradiation.
开发了一种简单的表面增强拉曼光谱（SERS）微流控池，用于研究从含有石内蓝细菌的北极沙漠采集的岩石样本中蓝细菌内scytonemin色素的分布。该检测方法在包括天体生物学和偏远环境中微生物分析在内的各种应用中具有潜在的未来用途，涉及研究地质样本中细菌的SERS光谱。通过在微流控装置中使用分散的胶体基质，与天然拉曼光谱相比，在测定微生物中的色素时获得了大于10(5)的表面增强。对scytonemin具有纳摩尔灵敏度的SERS检测表明，由于高剂量紫外线照射，在经历最高压力环境的样本中色素浓度最高。
