Du Huifang, Yan Huifang, Li Ye, Wang Juan
Institute of Occupational Health and Poisoning Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2007 May;36(3):369-71.
To develop a simple and sensitive GC/MS method for the determination of 4-nonylphenol (4-NP) and bisphenol A (BPA) from vegetables.
After smashed, the samples were extracted using ultrasonic extraction, and CH cartridge was used to clean the impurity, then desorbed with dichloromethane, the elution was chemically dried with anhydrous sodium sulphate and a silylation step was carried out with N,O-bis(trimethylsilyl) -trifluoroacetamide (BSTFA) to enhance the selectivity and sensitivity of the detection. Then the derivation were identified and quantified by GC-MS.
The linear range is from 10 to 500 microg/L for two target compounds with the correlation coefficient of 0.999, and the limit of detection(LOD) is 0.1microg/kg (4-NP) and 0.02 microg/kg (BPA), respectively. The spiked recoveries of two target compounds are ranged from 85.0% - 105.1%, and the precision are both below 10%.
This method has fairly high sensitivity and good precision, it can be used to detect the trace amount of 4-NP and BPA from vegetables.
建立一种简单、灵敏的气相色谱/质谱法（GC/MS）测定蔬菜中的4-壬基酚（4-NP）和双酚A（BPA）。
样品粉碎后，采用超声提取，用CH柱净化杂质，然后用二氯甲烷解吸，洗脱液用无水硫酸钠进行化学干燥，并用N,O-双（三甲基硅基）-三氟乙酰胺（BSTFA）进行硅烷化步骤以提高检测的选择性和灵敏度。然后通过GC-MS对衍生物进行鉴定和定量。
两种目标化合物的线性范围为10至500μg/L，相关系数为0.999，检测限（LOD）分别为0.1μg/kg（4-NP）和0.02μg/kg（BPA）。两种目标化合物的加标回收率为85.0% - 105.1%，精密度均低于10%。
该方法具有较高的灵敏度和良好的精密度，可用于检测蔬菜中痕量的4-NP和BPA。
