Perea Vélez M, Hermans K, Verhoeven T L A, Lebeer S E, Vanderleyden J, De Keersmaecker S C J
Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Leuven, Belgium.
J Appl Microbiol. 2007 Sep;103(3):666-74. doi: 10.1111/j.1365-2672.2007.03294.x.
Considering the significant rise in the probiotic market in Columbia, and given the lack of reports concerning the microbial population and strain performance in products from different producers, this study aims at determining the number of viable starter bacteria and probiotics in bio-yoghurts available at the Columbian market, identifying the species and analysing the performance of the isolated strains in bile acid resistance, antagonistic activity against pathogens, and adherence capacity to human intestinal epithelial cells.
Seven bio-yoghurts were analysed for the bacterial species present. Species identification was carried out using 16S rRNA gene targeted PCR. The cultured bacteria were tested for bile acid resistance, adherence to a human intestinal epithelial cell line, and antagonism against the pathogen Salmonella enterica serovar Typhimurium. A total of 17 different strains were identified. Based on plate counting, all bio-yoghurts have at least total viable cells of approximately 10(7) CFU ml(-1). Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus were the most frequently isolated bacteria. Viable Bifidobacterium was only recovered from one product. However, after PCR analysis, DNA of this genus was confirmed in five out of seven products. Major differences were found for S. typhimurium antagonism. The adherence capacity to Caco-2 cells was observed in 10 of the isolated strains. In general, low survival to simulated gastric juice was observed.
Some of the isolated strains have probiotic potential, although not all of them were present in the advised amount to exert beneficial health effects. However, the full correct scientific name of the isolated bacteria and their viable counts were not included on the product label.
This is the first report describing the identification and functionality of starter bacteria and probiotics present in dairy products on the Columbian market.
鉴于哥伦比亚益生菌市场的显著增长，且缺乏关于不同生产商产品中微生物种群和菌株性能的报告，本研究旨在确定哥伦比亚市场上可得的生物酸奶中活的发酵剂细菌和益生菌的数量，鉴定其种类，并分析分离菌株在胆汁酸抗性、对病原体的拮抗活性以及对人肠上皮细胞的黏附能力方面的性能。
对七种生物酸奶进行了细菌种类分析。使用靶向16S rRNA基因的PCR进行物种鉴定。对培养的细菌进行胆汁酸抗性、对人肠上皮细胞系的黏附以及对病原体鼠伤寒沙门氏菌的拮抗作用测试。共鉴定出17种不同菌株。基于平板计数，所有生物酸奶的总活细胞数至少约为10(7) CFU ml(-1)。嗜热链球菌和德氏保加利亚乳杆菌是最常分离出的细菌。仅从一种产品中回收了活的双歧杆菌。然而，经过PCR分析，在七种产品中的五种中确认了该属的DNA。在对鼠伤寒沙门氏菌的拮抗作用方面发现了主要差异。在10株分离菌株中观察到了对Caco-2细胞的黏附能力。总体而言，观察到对模拟胃液的低存活率。
一些分离菌株具有益生菌潜力，尽管并非所有菌株的数量都达到发挥有益健康作用的建议量。然而，产品标签上未包含分离细菌的完整正确学名及其活菌计数。
这是第一份描述哥伦比亚市场上乳制品中发酵剂细菌和益生菌的鉴定及功能的报告。
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