Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
Department of Medicine, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan.
BMC Microbiol. 2024 Aug 22;24(1):310. doi: 10.1186/s12866-024-03464-5.
Bovine mastitis results in significant economic losses for the dairy industry globally due to milk production losses and decreased herd efficiency. This research aimed to isolate, select, and characterize indigenous lactobacilli with probiotic properties. A total of 40 lactobacilli were isolated from healthy milk samples of cattle and identified at the species level through 16S rDNA sequencing. All isolates were initially screened for antimicrobial activity, and selected isolates underwent in vitro assessment of probiotic properties.
Among the lactobacilli isolates, varying levels of activity (9 to 19 mm) against cattle mastitogens; Stapylococcus aureus (Staph. aureus), Escherichia coli (E. coli) and Streptococcus dysgalactiae (Strep. dysgalactiae) were observed in the well diffusion assay. These isolates demonstrated auto-aggregation (ranging from 14.29 ± 0.96% to 62.11 ± 1.09%) and co-aggregate (ranging from 9.21 ± 0.14% to 55.74 ± 0.74%) with mastitogens after 2 h. Lactobacillus (Lb.) plantarum CM49 showed sensitivity to most antibiotics tested and exhibited strong inhibitory effects, with mean log10 reductions of 3.46 for Staph. aureus, 2.82 for E. coli, and 1.45 for Strep. dysgalactiae in co-culture experiments. Furthermore, Lb. plantarum CM49 significantly decreased the adhesion rate of mastitogens on the bovine mammary cell line and mouse model, demonstrating its potential effectiveness in preventing mastitis.
It is concluded that Lb. plantarum CM49 has remarkable probiotic potential with activity against cattle mastitogens in the laboratory and cell culture and competitively excludes mastitogens from bovine mammary cells and ameliorates Staph. aureus-induced mastitis in mice.
牛乳腺炎导致全球奶制品行业遭受重大经济损失，原因是产奶量下降和牛群效率降低。本研究旨在分离、选择和鉴定具有益生菌特性的本土乳杆菌。从健康牛奶样中分离出 40 株乳杆菌，并通过 16S rDNA 测序鉴定为种水平。所有分离株均进行了初始抗菌活性筛选，并对选定的分离株进行了体外益生菌特性评估。
在乳杆菌分离株中，通过平板打孔法观察到对牛乳腺炎病原体金黄色葡萄球菌（Staph. aureus）、大肠杆菌（E. coli）和无乳链球菌（Strep. dysgalactiae）的活性（9-19mm）存在差异。这些分离株在 2 小时后与乳腺炎病原体表现出自动聚集（范围为 14.29±0.96%至 62.11±1.09%）和共聚（范围为 9.21±0.14%至 55.74±0.74%）。植物乳杆菌 CM49 对大多数测试抗生素敏感，并表现出较强的抑制作用，在共培养实验中，对金黄色葡萄球菌的平均对数减少 3.46，对大肠杆菌的平均对数减少 2.82，对无乳链球菌的平均对数减少 1.45。此外，植物乳杆菌 CM49 显著降低了乳腺炎病原体在牛乳腺细胞系和小鼠模型上的黏附率，表明其在预防乳腺炎方面具有潜在的有效性。
植物乳杆菌 CM49 具有显著的益生菌潜力，在实验室和细胞培养中对牛乳腺炎病原体具有活性，在体外竞争排除乳腺炎病原体与牛乳腺细胞的黏附，并改善金黄色葡萄球菌诱导的小鼠乳腺炎。
