Rahmati Farzad
Department of Microbiology, Faculty of Science, Qom Branch, Islamic Azad University, Qom, IR Iran.
AIMS Microbiol. 2017 Oct 12;3(4):815-825. doi: 10.3934/microbiol.2017.4.815. eCollection 2017.
The aim of the present study was to investigate technological properties of starter strains from traditional dairy products collected from five villages of Lorestan province in Iran. Thirty five samples were cultured on selective media (MRS broth, Nutrient Broth and YGC and then typical colonies checked for morphological features and eventually eighty two strains selected for further examination. The strains were evaluated for Hydrolysis of casein, starch and citrate, growth at 15 and 45 °C, growth in 4 and 6.5% NaCl, resistance to antibiotics (ampicillin, bacitracin, chloramphenicol, erythromycin, gentamicin, penicillin, novobiocin, nalidixic acid) proteolytic and lipolytic and acidification activities. Sixteen strains chosen according to the difference in cell morphology and were identified using API galleries and ability to metabolize various carbohydrates, which consequently, led to identifying seven , five , two and two In general, two strains of AKL2, DDL2, two strains of SYL5, ACL4 and one strain of DDy2 was demonstrated the most important technological characterization that suitable for using as starter cultures.
本研究的目的是调查从伊朗洛雷斯坦省五个村庄收集的传统乳制品中发酵剂菌株的技术特性。将35个样品在选择性培养基(MRS肉汤、营养肉汤和YGC)上培养,然后检查典型菌落的形态特征,最终选择82株菌株进行进一步检测。对这些菌株进行了酪蛋白、淀粉和柠檬酸盐水解、在15和45°C下生长、在4%和6.5%氯化钠中生长、对抗生素(氨苄青霉素、杆菌肽、氯霉素、红霉素、庆大霉素、青霉素、新生霉素、萘啶酸)的抗性、蛋白水解和脂肪分解以及酸化活性的评估。根据细胞形态的差异选择了16株菌株,并使用API鉴定库以及代谢各种碳水化合物的能力进行鉴定,结果鉴定出7株、5株、2株和2株。总体而言,两株AKL2、DDL2,两株SYL5、ACL4以及一株DDy2表现出最适合用作发酵剂培养物的重要技术特性。