Morris R E, Fritz R B
Cancer Res. 1976 Feb;36(2 Pt 1):414-9.
The immunological properties of the surface of chick embryo cells infected with strain MC29 avian leukosis virus were investigated by immune electron microscopy in conjunction with antiviral and anticellular immune sera. When samples were taken at sequential times after infection, it was found that cells stained strongly for viral antigen very early after infection. Staining reached a minimum 6 to 12 hr after infection and then increased to maximum at 15 hr after infection, remaining at this level at later times. Anticellular immune sera showed the presence of a distinct antigen or antigens which appeared concurrently with morphological alteration of the infected chick cells. This antigen was cross-reactive or identical to an antigen appearing on the surface of chick embryo cells transformed by the Schmidt-Ruppin strain of Rous sarcoma virus.
利用免疫电子显微镜结合抗病毒和抗细胞免疫血清，研究了感染MC29株禽白血病病毒的鸡胚细胞表面的免疫特性。当在感染后的连续时间点取样时，发现感染后很早细胞就被强烈染色以显示病毒抗原。染色在感染后6至12小时达到最低水平，然后在感染后15小时增加到最高水平，并在随后的时间保持在该水平。抗细胞免疫血清显示存在一种独特的抗原，该抗原与感染的鸡细胞的形态改变同时出现。这种抗原与由劳斯肉瘤病毒施密特-鲁平株转化的鸡胚细胞表面出现的一种抗原交叉反应或相同。
