Gogusev J, Suskind G, Gilmour D, Haguenau F, Teutsch B, Hlozánek I, Rabotti G F
Laboratoire de Médecine expérimentale, Collège de France, Paris.
Folia Biol (Praha). 1987;33(5):325-34.
Monoclonal monomorphic as well as polyclonal antibodies against H-B class I (B-F) antigen were used to evaluate the expression of these molecules on normal and RSV-transformed chick embryo fibroblasts. The results indicate that chicken fibroblasts transformed in vitro by the Schmidt-Ruppin strain Rous sarcoma virus of subgroup B (SR-RSV-B) present reduced H-B class I antigen expression as compared to uninfected cells of the same inbred strain. This quantitative reduction was observed at the cell membrane and in whole cell lysates. Chick embryo fibroblasts infected with avian leukosis virus RAV-1 show that the levels of H-B class I antigens were indistinguishable from the levels measured on the control cells. Normal embryo fibroblasts derived from the inbred lines B4/B4 express higher levels of H-B antigens than normal cells carrying the B12/B12 genotype.
使用针对H-B I类（B-F）抗原的单克隆单态以及多克隆抗体，来评估这些分子在正常和经呼吸道合胞病毒（RSV）转化的鸡胚成纤维细胞上的表达。结果表明，与同一近交系未感染细胞相比，由B亚群施密特-鲁平株劳氏肉瘤病毒（SR-RSV-B）体外转化的鸡成纤维细胞呈现出H-B I类抗原表达降低。在细胞膜和全细胞裂解物中均观察到这种定量减少。感染禽白血病病毒RAV-1的鸡胚成纤维细胞显示，H-B I类抗原水平与对照细胞上测得的水平无差异。源自近交系B4/B4的正常胚成纤维细胞比携带B12/B12基因型的正常细胞表达更高水平的H-B抗原。
