Andersen G E, Nielsen H G
Clin Chim Acta. 1976 Jan 2;66(1):29-41. doi: 10.1016/0009-8981(76)90368-5.
The early detection of hyperlipoproteinemia in newborn infants has so far been based upon estimation of cord blood total lipids (cholesterol and triglyceride) and lipoprotein-lipids (VLDL-, LDL- and HDL-cholesterol). To be able to make a direct estimation of cord serum beta-lipoproteins (VLDL + LDL) two quite different methods were modified, one immunological and the other turbidimetric. Good correlations were found to VLDL- + LDL-cholesterol isolated in the ultracentrifuge (r = 0.848 and 0.831, respectively). If neonatal screening for hyperlipoproteinemia is considered, we recommend the very easy and inexpensive turbidimetric method. Furthermore, using cord serum, two conventional precipitation methods with heparin-CaCl2 and heparin-MnCl2 were compared by ultracentrifugation and high correlations were found (r = 0.923 and 0.899, respectively). A clamping study showed that following early clamping of the cord, the concentration of cord serum lipids and lipoproteins did not change markedly within the first five minutes. Storing experiments showed that serum should be separated within the first 12 h to avoid unpredictable changes in the concentration of cord serum lipids and lipoproteins.
迄今为止，新生儿高脂血症的早期检测一直基于对脐血总脂质（胆固醇和甘油三酯）以及脂蛋白脂质（极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白胆固醇）的评估。为了能够直接估算脐血清β脂蛋白（极低密度脂蛋白+低密度脂蛋白），对两种截然不同的方法进行了改进，一种是免疫法，另一种是比浊法。发现与超速离心分离出的极低密度脂蛋白+低密度脂蛋白胆固醇有良好的相关性（分别为r = 0.848和0.831）。如果考虑对新生儿进行高脂血症筛查，我们推荐非常简便且廉价的比浊法。此外，使用脐血清，通过超速离心比较了两种常规的肝素 - 氯化钙和肝素 - 氯化锰沉淀法，发现有高度相关性（分别为r = 0.923和0.899）。一项钳夹研究表明，在早期钳夹脐带后，脐血清脂质和脂蛋白的浓度在最初五分钟内没有明显变化。储存实验表明，血清应在最初12小时内分离，以避免脐血清脂质和脂蛋白浓度出现不可预测的变化。
