Chen Wenjin, Foran David J
The Center for Biomedical Imaging & Informatics, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, NJ 08854, United States.
Anal Chim Acta. 2006 Mar 30;564(1):74-81. doi: 10.1016/j.aca.2005.11.083. Epub 2006 Jan 23.
Over the past few years, tissue microarray (TMA) technology has been established as a standard method for assessing the expression of proteins or genes across large sets of tissue specimens. It is being adopted increasingly among leading research institutions around the world and utilized in cancer research in parallel with the cDNA microarray technology. This article summarizes various aspects of cancer understanding and diagnostics in which TMA has had great impact. Although tremendous advances continue to be made to facilitate imaging and archiving of TMA specimens, automatic evaluation and quantitative analysis of TMA still remains an important challenge for modern investigators.
在过去几年中,组织微阵列(TMA)技术已成为评估大量组织标本中蛋白质或基因表达的标准方法。它在世界各地的顶尖研究机构中越来越多地被采用,并与cDNA微阵列技术并行应用于癌症研究。本文总结了TMA在癌症理解和诊断的各个方面所产生的重大影响。尽管在促进TMA标本的成像和存档方面不断取得巨大进展,但TMA的自动评估和定量分析对现代研究人员来说仍然是一项重大挑战。