利用激光诱导荧光光谱法分析绘画作品中基于蛋白质的结合介质。

Analysis of protein-based binding media found in paintings using laser induced fluorescence spectroscopy.

作者信息

Nevin Austin, Cather Sharon, Anglos Demetrios, Fotakis Costas

机构信息

Conservation of Paintings Department, Courtauld Institute of Art, University of London, Somerset House, Strand, WC2R ORN London, UK.

出版信息

Anal Chim Acta. 2006 Jul 28;573-574:341-6. doi: 10.1016/j.aca.2006.01.027. Epub 2006 Feb 14.

DOI:10.1016/j.aca.2006.01.027

PMID:17723543

Abstract

Laser induced fluorescence (LIF) spectroscopy of intrinsic fluorophores from organic media found in paintings (casein, animal glue and egg proteins) provides novel non-invasive means of characterisation of general classes of media on the basis of fluorescence emission arising from the presence of certain amino acids and their degradation byproducts. Proteins from traditionally employed binding media include collagen, casein, albumin and other egg proteins, of animal sources (skins, milk and egg respectively). Wavelength dependence of the spectra is presented for analyses of thin films of protein-based binding media.

摘要

对绘画中发现的有机介质（酪蛋白、动物胶和蛋清蛋白）的固有荧光团进行激光诱导荧光（LIF）光谱分析，基于某些氨基酸及其降解副产物产生的荧光发射，提供了一种新型的非侵入性介质分类表征方法。传统使用的粘结介质中的蛋白质包括动物来源（分别为皮肤、牛奶和鸡蛋）的胶原蛋白、酪蛋白、白蛋白和其他蛋清蛋白。给出了光谱的波长依赖性，用于分析基于蛋白质的粘结介质薄膜。