Wang Hai-Qiao, Liu Tian-Cai, Cao Yuan-Cheng, Huang Zhen-Li, Wang Jian-Hao, Li Xiu-Qing, Zhao Yuan-Di
Key Laboratory of Biomedical Photonics of Ministry of Education-Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China.
Anal Chim Acta. 2006 Oct 27;580(1):18-23. doi: 10.1016/j.aca.2006.07.048. Epub 2006 Jul 26.
A flow cytometric detecting technology based on quantum dots (QDs)-encoded beads has been described. Using this technology, several QDs-encoded beads with different code were identified effectively, and the target molecule (DNA sequence) in solution was also detected accurately by coupling to its complementary sequence probed on QDs-encoded beads through DNA hybridization assay. The resolution of this technology for encoded beads is resulted from two longer wavelength fluorescence identification signals (yellow and red fluorescent signals of QDs), and the third shorter wavelength fluorescence signal (green reporting signal of fluorescein isothiocyanate (FITC)) for the determination of reaction between probe and target. In experiment, because of QDs' unique optical character, only one excitation light source was needed to excite the QDs and probe dye FITC synchronously comparing with other flow cytometric assay technology. The results show that this technology has present excellent repeatability and good accuracy. It will become a promising multiple assay platform in various application fields after further improvement.
一种基于量子点（QDs）编码微球的流式细胞检测技术已被报道。利用该技术，能够有效识别几种具有不同编码的QDs编码微球，并且通过DNA杂交分析，将溶液中的靶分子（DNA序列）与其在QDs编码微球上探测到的互补序列偶联，也能准确检测到靶分子。该技术对编码微球的分辨率源于两个较长波长的荧光识别信号（QDs的黄色和红色荧光信号），以及用于确定探针与靶标之间反应的第三个较短波长荧光信号（异硫氰酸荧光素（FITC）的绿色报告信号）。在实验中，由于QDs独特的光学特性，与其他流式细胞检测技术相比，仅需一个激发光源就能同时激发QDs和探针染料FITC。结果表明，该技术具有出色的重复性和良好的准确性。经过进一步改进后，它将成为一个在各种应用领域中颇具前景的多重检测平台。
