Gómez-Hens A, Fernández-Romero J M, Aguilar-Caballos M P
Department of Analytical Chemistry, "Marie Curie Annex" Building, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain.
Trends Analyt Chem. 2008 May;27(5):394-406. doi: 10.1016/j.trac.2008.03.006. Epub 2008 Mar 31.
We critically evaluate the usefulness of different nanostructures described as labels, nanoscaffolds or separation media in immunoassays and nucleic-acid hybridization assays. Many of the great number of publications describe only theoretical aspects of using these nanostructures or nanoparticles, but do not verify their applicability in the presence of potential interferents that can be present in the sample matrix. We attempt a systematic study of the advantages and the limitations of using these new reagents in bioassays, the different assay formats for individual and multiplexed detection, and the capability of these assays in analyzing real samples.
我们严格评估了不同纳米结构在免疫测定和核酸杂交测定中作为标记物、纳米支架或分离介质的实用性。大量出版物中的许多仅描述了使用这些纳米结构或纳米颗粒的理论方面，但未在可能存在于样品基质中的潜在干扰物存在的情况下验证其适用性。我们尝试对在生物测定中使用这些新试剂的优点和局限性、用于个体和多重检测的不同测定形式以及这些测定分析实际样品的能力进行系统研究。
