Girelli Anna Maria, Mattei Enrico, Messina Antonella
Dipartimento di Chimica, Università degli Studi di Roma "La Sapienza", P.le Aldo Moro 5, 00185 Roma, Italy.
Anal Chim Acta. 2006 Nov 24;580(2):271-7. doi: 10.1016/j.aca.2006.07.088. Epub 2006 Aug 12.
The development of an immobilized enzyme reactor (IMER) based on tyrosinase immobilized on aminopropyl-controlled pore glass (AP-CPG) for the removal of phenols from model aqueous solutions was reported. To elucidate the influence of the substrate nature, the apparent (V'max, K'm) and the inherent (Vmax, Km) Michaelis-Menten constants were determined by Lineweaver-Burk method and the external diffusional contributions on measured enzyme activities were removed by a graphical method. The dephenolization process was realized by recycling the phenol solutions through the bioreactor connected to a chitosan trap in order to remove the colored quinone-type products of the tyrosinase reactions. The results indicated that a complete removal of phenol derivatives in the range of 150-300 min, with the exception of 60% removal for phenol reached in 400 min, was obtained. The observed sequence: cresol > 4-methylcathecol > catechol > 4-Cl-phenol phenol was in accordance to the V'max/K'm values.
报道了一种基于固定在氨丙基可控孔径玻璃（AP-CPG）上的酪氨酸酶的固定化酶反应器（IMER），用于从模拟水溶液中去除酚类物质。为了阐明底物性质的影响，通过Lineweaver-Burk方法测定了表观（V'max，K'm）和固有（Vmax，Km）米氏常数，并通过图形方法消除了外部扩散对测得的酶活性的贡献。脱酚过程是通过将酚溶液循环通过连接到壳聚糖捕集器的生物反应器来实现的，以去除酪氨酸酶反应产生的有色醌类产物。结果表明，除了在400分钟内对苯酚的去除率达到60%外，在150-300分钟范围内可实现酚类衍生物的完全去除。观察到的顺序：甲酚>4-甲基邻苯二酚>邻苯二酚>4-氯苯酚>苯酚，与V'max/K'm值一致。
