Thomson G R, Mumford J A, Campbell J, Griffiths L, Clapham P
Equine Vet J. 1976 Apr;8(2):58-65. doi: 10.1111/j.2042-3306.1976.tb03291.x.
An investigation was made of 3 serological tests (virus neutralization, complement fixation and indirect immunofluorescence), which are applicable to epidemiological studies of infections by Equid herpesvirus 1 (EHV-1). Sera from gnotobiotic foals inoculated intranasally with various strains of EHV-1 were unable in some cases to neutralize heterologous strains and these results were not consistent with the existence of clearly-defined subtypes of EHV-1, as previously proposed. The cross-reactions in complement-fixation tests paralleled those with neutralization but immunofluorescence tests were found to be both more sensitive and more broadly reactive than the other two. Complement-fixing antibodies declined more rapidly following experimental infection than did those measured by neutralization or immunofluorescence. The results are discussed in relation to the diagnosis of EHV-1 infection and the significance they may have for the epidemiology of this disease.
对三种血清学检测方法（病毒中和试验、补体结合试验和间接免疫荧光试验）进行了研究，这些方法适用于马疱疹病毒1型（EHV-1）感染的流行病学研究。经鼻接种不同EHV-1毒株的无菌幼驹的血清，在某些情况下无法中和异源毒株，这些结果与先前提出的EHV-1存在明确定义的亚型不一致。补体结合试验中的交叉反应与中和试验中的交叉反应相似，但发现免疫荧光试验比其他两种试验更敏感且反应更广泛。实验感染后，补体结合抗体的下降速度比中和试验或免疫荧光试验所检测的抗体下降速度更快。结合EHV-1感染的诊断以及这些结果对该疾病流行病学的意义进行了讨论。
