青霉素G酰化酶基因在巨大芽孢杆菌中的克隆与表达
Cloning and expression of penicillin G acylase gene in Bacillus megaterium.
作者信息
Zhang L F, Li Z W, Zhang Q J
机构信息
Institute of Biophysics, Chinese Academy of Sciences, Beijing.
出版信息
Chin J Biotechnol. 1991;7(1):63-72.
Bacillus megaterium BM1, which produces penicillin G acylase (PGA), has been isolated. Gene encoding for PGA was cloned into E. coli MC1061 using pBR322 as the vector, obtaining a recombinant plasmid pBmPA4 containing 9.9 kb inserted DNA. Restriction map of the plasmid was analyzed. A pBmPA5 containing 4.9 kb was gained by deletion in vitro. Both pBmPA4 and pBmPA5 clones can be expressed in E.coli MC1061, and their expressions were induced by phenylacetic acid.
已分离出能产生青霉素G酰化酶（PGA）的巨大芽孢杆菌BM1。使用pBR322作为载体，将编码PGA的基因克隆到大肠杆菌MC1061中，获得了一个含有9.9 kb插入DNA的重组质粒pBmPA4。分析了该质粒的限制性图谱。通过体外缺失获得了一个含有4.9 kb的pBmPA5。pBmPA4和pBmPA5克隆均可在大肠杆菌MC1061中表达，且它们的表达由苯乙酸诱导。
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