Katayama Y, Tsubokawa T, Koshinaga M, Miyazaki S
Department of Neurological Surgery, Nihon University School of Medicine, Tokyo, Japan.
Brain Res. 1991 Oct 25;562(2):352-5. doi: 10.1016/0006-8993(91)90647-e.
Cell suspensions obtained from the fetal hippocampus were transplanted into the adult rat hippocampus at 1 or 4 weeks after transient forebrain ischemia. Only when the ischemia induced death of most of the CA1 pyramidal cells of the host hippocampus and transplantation was performed at 1 week after the ischemia, did a large number of transplanted cells survive and the most extensive dendritic growth was demonstrated by microtubule-associated protein 2 immunohistochemistry. The dendrites of the cells located in the ventral part were oriented ventrally, lining up similarly to the parallel arrangements of apical dendrites of normal CA1 pyramidal cells. These findings suggest that certain forms of trophic factors, which appear to occur in association with the presence of free terminals of afferent fibers during the earlier period after ischemic insult, are involved in the survival of and dendritic growth from transplanted hippocampal cells.
将从胎鼠海马体获得的细胞悬液，在短暂性前脑缺血后1周或4周移植到成年大鼠海马体中。只有当缺血导致宿主海马体大部分CA1锥体细胞死亡且在缺血后1周进行移植时，大量移植细胞才能存活，并且通过微管相关蛋白2免疫组化显示出最广泛的树突生长。位于腹侧的细胞的树突向腹侧定向，排列方式类似于正常CA1锥体细胞顶树突的平行排列。这些发现表明，某些形式的营养因子似乎在缺血性损伤后的早期与传入纤维的游离终末的存在相关联，它们参与移植海马体细胞的存活和树突生长。
