Science. 1946 Nov 22;104(2708):488-90. doi: 10.1126/science.104.2708.488.
A rapid, simple, quantitative technique has been developed for the study of the action of hydrolytic enzymes on certain insoluble substrates. The end point of digestion is indicated by the release of a glass bead, when two pieces of lens paper sealed together by the substrate separate due to its digestion by the enzyme.
现已开发出一种快速、简单、定量的技术,用于研究水解酶对某些不溶性底物的作用。消化终点由玻璃珠的释放来指示,当被底物密封在一起的两片透镜纸因酶的消化而分离时,玻璃珠释放出来。