Van Baalen C
Science. 1965 Jul 2;149(3679):70. doi: 10.1126/science.149.3679.70.
Cells of Anacystis nidulans in the logarithmic phase of growth were briefly treated with the mutagen, N-methyl-N'-nitro-N-nitrosoguanidine, and plated under conditions suitable for single-cell growth. Selection of aberrant colonies and examination of the cultural characteristics of these clones suggest that mutants of at least several types are easily derivable in Anacystis.
处于对数生长期的集胞藻细胞用诱变剂N-甲基-N'-硝基-N-亚硝基胍进行短暂处理,然后在适合单细胞生长的条件下进行平板接种。对异常菌落的筛选以及对这些克隆的培养特征的检查表明,在集胞藻中至少可以很容易地获得几种类型的突变体。