蓝绿藻集胞藻6803尿嘧啶营养缺陷型的分离与初步鉴定
Isolation and initial characterization of a uracil auxotroph of the blue-green alga Anacystis nidulans.
作者信息
Stevens C L, Stevens S E, Myers J
出版信息
J Bacteriol. 1975 Oct;124(1):247-51. doi: 10.1128/jb.124.1.247-251.1975.
Abstract
A uracil-requiring auxotroph of Anacystis nidulans was isolated after treatment with N-methyl-N'-nitrosoguanidine. Neither precursors in the de novo pyrimidine pathway nor compounds of "salvage" or degradative pathways could replace the uracil requirement. The reversion frequency for mutation to a nonuracil requirement for growth was 2.0 times 10(-8). The calculated average rate of uracil utilization was 1.1 times 10(-17) mol of uracil per unit cell mass/h. The amount of uracil required for the synthesis of a unit cell mass was 3.8 times 10(-17) mol of uracil.
摘要
在用N-甲基-N'-亚硝基胍处理后，分离得到了集胞藻（Anacystis nidulans）的一株尿嘧啶营养缺陷型菌株。从头嘧啶途径的前体物质、“补救”途径或降解途径的化合物均不能替代对尿嘧啶的需求。向不依赖尿嘧啶生长的突变回复频率为2.0×10⁻⁸。计算得出的尿嘧啶平均利用速率为每单位细胞质量每小时1.1×10⁻¹⁷摩尔尿嘧啶。合成单位细胞质量所需的尿嘧啶量为3.8×10⁻¹⁷摩尔尿嘧啶。
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