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[蓝绿藻集胞藻(聚球藻)不同生长阶段的特征]

[Characteristics of different growth stages of the blue-green alga Anacystis nidulans (Synechococcus)].

作者信息

Nikitina K A, Gusev M V

出版信息

Mikrobiologiia. 1976 May-Jun;45:490-6.

PMID:826762
Abstract

The rate of growth of the blue-green alga Anacystis nidulans is highest during the first 5--8 days but the number of the cells increases up to 30 days when the stationary phase takes place (30--60 days). The content of chlorophyll is almost constant during the first 20 days of growth while the content of phycocyanin increases; then the content of the both pigments decreases. The rate of O2 evolution and the rate of electron transport to ferricyanide decrease exponentionally during the first 10 days of growth. The rate of endogenous respiration and the rate of O2 absorption, induced by methylviologen in the light in the presence of DCMU decrease more slowly. At the same time, the rate of O2 absorption in the presence of methylviologen without DCMU and the rate of TTC reduction in the darkness increase and later decrease. The rate of TTC reduction in the light increases up to 30--40 days and then decreases. The rate of the cell death in the darkness decreases when the age of the "light" culture is 50 days. Addition to the medium of its main components does not increase the rate of growth of the culture after 10 days of its growth. Addition of CaCl2, glucose, and asparagic acid increases the rate of growth of the 3-day-old culture. Addition of phosphorus inhibits growth. Argon favours growth of the culture. The cells are rapidly discoloured in conditions of hampered gas exchange; they die rapidly also in the presence of DCMU, methylviologen, and KCN.

摘要

蓝绿藻集胞藻的生长速率在前5 - 8天最高,但细胞数量在30天内持续增加,之后进入稳定期(30 - 60天)。叶绿素含量在生长的前20天几乎保持恒定,而藻蓝蛋白含量增加;随后两种色素的含量均下降。在生长的前10天,氧气释放速率和向铁氰化物的电子传递速率呈指数下降。内呼吸速率以及在DCMU存在下,甲基紫精在光照下诱导的氧气吸收速率下降得更慢。同时,在无DCMU的情况下,甲基紫精存在时的氧气吸收速率以及黑暗中TTC还原速率先增加后下降。光照下TTC还原速率在30 - 40天内增加,然后下降。当“光照”培养物培养50天时,黑暗中细胞死亡速率下降。培养10天后,向培养基中添加其主要成分不会提高培养物的生长速率。添加氯化钙、葡萄糖和天冬氨酸可提高3日龄培养物的生长速率。添加磷会抑制生长。氩气有利于培养物的生长。在气体交换受阻的条件下,细胞迅速褪色;在DCMU、甲基紫精和KCN存在的情况下,细胞也会迅速死亡。

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