Science. 1965 Jul 2;149(3679):72-3. doi: 10.1126/science.149.3679.72.
Certain problems usually associated with the histochemistry of lipases are obviated by a technique that utilizes the endogenous blood chylomicrons and the cellular stores of triglyceride as substrates for the histochemical demonstration of lipolytic enzyme activity in situ. In spreads of mesenteric adipose tissue, the technique makes it possible to distinguish between lipoprotein lipase activity at sites in the capillaries and lipolysis occurring in the adipocytes. The selective anatomic lolization of the lipase reaction correlated with the functional state of the tissue, and the absence of reaction product in control mesenteries from starved mice or in heat-inactivated controls, support the validity of this histochemical reaction.
利用内源性血乳糜微粒和细胞内甘油三酯储存作为底物的技术可以避免脂肪酶组织化学中通常出现的某些问题，原位显示脂肪酶活性。在肠系膜脂肪组织的铺片中，该技术可区分毛细血管部位的脂蛋白脂肪酶活性和脂肪细胞中的脂肪分解。脂肪酶反应的选择性解剖定位与组织的功能状态相关，并且在饥饿小鼠或热失活对照物的肠系膜对照物中没有反应产物，支持了这种组织化学反应的有效性。
