Hausman G J, Novakofski J E, Martin R J, Thomas G B
Cell Tissue Res. 1984;236(2):459-64. doi: 10.1007/BF00214250.
Primary cultures of stromal-vascular cells of adipose tissue from fetuses at 70 and 110 days of gestation were evaluated as potential model systems for studies of fetal adipocyte differentiation and proliferation. In the cultures, fat cells developed as very discrete clusters. Fat cell cluster development was dependent on initial cell density and time. Histochemical analysis for NADP-dependent dehydrogenases revealed an age of donor effect. Similar levels of enzymes (malate and glucose-6-phosphate dehydrogenase) were apparent in fat cell clusters and stromal cells in cultures of cells from fetuses at 70 days of gestation. These enzymes were only present in fat cell clusters in cultures of cells from fetuses at 110 days of gestation. The distribution of histochemically detectable esterase activity was dependent on the cell density at time of analysis. In areas of high cell density, esterase was restricted to fat cell clusters whereas, both stromal cells and fat cells were esterase reactive in areas of low cell density. Omitting PMS from the dehydrogenase media revealed differences in enzyme reactions of cells grown on collagen-coated and uncoated glass surfaces. These studies demonstrate that primary cultures of stromal-vascular cells from 110-day-old fetuses would be a useful system to identify factors involved in adipocyte proliferation and differentiation.
对妊娠70天和110天胎儿脂肪组织的基质血管细胞原代培养物进行了评估，将其作为研究胎儿脂肪细胞分化和增殖的潜在模型系统。在培养物中，脂肪细胞以非常离散的簇状形式发育。脂肪细胞簇的发育取决于初始细胞密度和时间。对NADP依赖性脱氢酶的组织化学分析显示了供体年龄效应。在妊娠70天胎儿的细胞培养物中，脂肪细胞簇和基质细胞中酶（苹果酸和葡萄糖-6-磷酸脱氢酶）的水平相似。这些酶仅存在于妊娠110天胎儿的细胞培养物中的脂肪细胞簇中。组织化学可检测的酯酶活性分布取决于分析时的细胞密度。在高细胞密度区域，酯酶仅限于脂肪细胞簇，而在低细胞密度区域，基质细胞和脂肪细胞均具有酯酶活性。从脱氢酶培养基中省略PMS显示了在胶原包被和未包被的玻璃表面上生长的细胞的酶反应差异。这些研究表明，来自110日龄胎儿的基质血管细胞原代培养物将是鉴定参与脂肪细胞增殖和分化的因素的有用系统。
