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从狍(Capreolus capreolus)克隆的串联重复序列的特征及其在两种麂属物种中的染色体定位

Characterisation of a tandem repetitive sequence cloned from the deer Capreolus capreolus and its chromosomal localisation in two muntjac species.

作者信息

Scherthan H

机构信息

Division of Human Biology and Human Genetics, University of Kaiserslautern, Germany.

出版信息

Hereditas. 1991;115(1):43-9. doi: 10.1111/j.1601-5223.1991.tb00345.x.

Abstract

The isolation and characterisation of a highly repetitive DNA sequence from the genome of the Roe deer Capreolus capreolus is reported. This sequence is characterised by tandem repetition and located within centric heterochromatin as demonstrated by non isotopic in situ hybridisation to the karyotypes of the Indian and Chinese muntjacs. Amplification and/or clustering of these sequences during the drastic karyotype evolution of the genus Muntiacus was noted in the large centromere of the X chromosome of the Indian muntjac. Partial sequence analysis revealed a 62% sequence homology with the sat 1A sequences of Muntiacus muntjak vaginalis.

摘要

报道了从狍(Capreolus capreolus)基因组中分离和鉴定出一种高度重复的DNA序列。该序列的特征是串联重复,通过对印度和中国麂的核型进行非同位素原位杂交证明其位于着丝粒异染色质内。在麂属剧烈的核型进化过程中,这些序列在印度麂X染色体的大着丝粒中出现了扩增和/或聚集现象。部分序列分析显示,它与黑麂(Muntiacus muntjak vaginalis)的sat 1A序列有62%的序列同源性。

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