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一种狍(Capreolus capreolus)特有的卫星DNA元件。

A satellite DNA element specific for roe deer (Capreolus capreolus).

作者信息

Buntjer J B, Nijman I J, Zijlstra C, Lenstra J A

机构信息

Institute of Infectious Diseases and Immunology, Utrecht University, The Netherlands.

出版信息

Chromosoma. 1998 Mar;107(1):1-5. doi: 10.1007/s004120050276.

Abstract

An abundant tandem repetitive DNA segment (CCsatIII) with a repeat unit of 2.2 kb has been found in the genome of roe deer (Capreolus capreolus). It accounts for approximately 5%-10% of the genome and is only present in the two species of the genus Capreolus. The sequence has no similarity or common motives with other deer satellite DNAs and there is no internal repeat structure. A 93 bp fragment is homologous to a bovine repeat. Fluorescent in situ hybridisation showed a predominant centromeric staining of most chromosomes accompanied by a weak interstitial staining of the same chromosomes. On Southern blots, CCsatIII probes do not discriminate between the closely related Capreolus species.

摘要

在狍(Capreolus capreolus)的基因组中发现了一个丰富的串联重复DNA片段(CCsatIII),其重复单元为2.2 kb。它约占基因组的5%-10%,且仅存在于狍属的两个物种中。该序列与其他鹿类卫星DNA没有相似性或共同基序,也没有内部重复结构。一个93 bp的片段与牛的重复序列同源。荧光原位杂交显示,大多数染色体的着丝粒染色占主导,同时相同染色体存在较弱的间质染色。在Southern杂交中,CCsatIII探针无法区分亲缘关系密切的狍属物种。

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