Anderson J L, Martin R G
J Cell Physiol. 1976 May;88(1):65-76. doi: 10.1002/jcp.1040880109.
Brain cells derived from the NIH Swiss mouse strain have been established in tissue culture. Astrocytic neuroglial cells, identified by morphology and staining properties, predominate. The brain cell culture was successfully transformed with SV40 wild type virus and with a representative early (A239) and late (C219) mutant. When subjected to growth analysis the A239 transformant displayed selective loss of six characteristics of the transformed phenotype at the restrictive temperature (40.5 degrees C): doubling time, saturation density, ability to grow in low serum, efficiency of growth on plastic and on normal brain cell layers, and cloning in soft agar. Temperature shift experiment demonstrated the reversibility of the differences in saturation density. T-antigen was expressed at both temperatures. Alteration in uptake of 2-deoxyglucose was not a characteristic of the transformed phenotype. The cell lines may be utility in brain culture work and in studies on the mechanism of SV40 transformation.
源自美国国立卫生研究院瑞士小鼠品系的脑细胞已在组织培养中建立。通过形态学和染色特性鉴定的星形胶质神经胶质细胞占主导。该脑细胞培养物已成功用SV40野生型病毒以及具有代表性的早期（A239）和晚期（C219）突变体进行转化。当进行生长分析时，A239转化体在限制温度（40.5摄氏度）下表现出转化表型的六个特征的选择性丧失：倍增时间、饱和密度、在低血清中生长的能力、在塑料和正常脑细胞层上的生长效率以及在软琼脂中的克隆能力。温度转换实验证明了饱和密度差异的可逆性。T抗原在两个温度下均有表达。2-脱氧葡萄糖摄取的改变不是转化表型的特征。这些细胞系可能在脑培养工作和SV40转化机制的研究中有用。
