Martin R G, Setlow V P, Edwards C A
J Virol. 1979 Sep;31(3):596-607. doi: 10.1128/JVI.31.3.596-607.1979.
Simian virus 40 mutants containing both a tsA mutation (rendering the 90,000 molecular weight [90K] T-antigen thermolabile) and a deletion between 0.54 and 0.59 map units (reducing the size and the amount of the 20K t-antigen) were used to transform Chinese hamster lung cells. The frequencies of transformation by the double mutants were comparable to that of the tsA mutant alone by both the focus and agar assays except when the cells were serum depleted before infection. Growth-arrested cells were transformed (using the agar assay) by the deletion mutants at less than 2% the frequency found when the 20K t-antigen was normal. Growth arrest had very little effect on the temperature sensitivity of the resultant transformed cell lines whether or not the deletion was present.
含有tsA突变（使90,000分子量[90K]的T抗原对热不稳定）和0.54至0.59图距单位之间缺失（减小20K t抗原的大小和数量）的猴病毒40突变体用于转化中国仓鼠肺细胞。通过焦点试验和琼脂试验，双突变体的转化频率与单独的tsA突变体相当，除非细胞在感染前血清耗尽。生长停滞的细胞被缺失突变体转化（使用琼脂试验）的频率不到20K t抗原正常时发现频率的2%。无论是否存在缺失，生长停滞对所得转化细胞系的温度敏感性影响很小。
