Bellon J, Weise B, Verschraegen G, De Meyere M
Department of Bacteriology, University Hospital, Ghent, Belgium.
J Clin Microbiol. 1991 Sep;29(9):2084-5. doi: 10.1128/jcm.29.9.2084-2085.1991.
In a study on acute pharyngitis in general practice, we compared a selective group A streptococcal agar (ssA) for the recovery of group A beta-hemolytic streptococci (GABHS) with sheep blood agar. All plates were incubated at 36 degrees C in an atmosphere reinforced with 5% CO2 for 48 h with a first reading after 24 h. A total of 197 GABHS isolates were obtained from 721 throat cultures on both media. The recovery of GABHS was significantly higher after 48 h of incubation for both media. With the ssA plate, we detected significantly more GABHS after 24 h as well as after 48 h of incubation. The ssA plate reduced normal flora qualitatively and quantitatively. In conclusion, ssA is more sensitive and specific for the detection of GABHS than sheep blood agar and moreover easier to read. We recommend incubation for 48 h.
在一项关于全科医疗中急性咽炎的研究中，我们将用于A组β溶血性链球菌（GABHS）分离的选择性A组链球菌琼脂（ssA）与羊血琼脂进行了比较。所有平板均在36℃、5%二氧化碳强化的气氛中培养48小时，24小时后进行首次读数。两种培养基上共从721份咽喉培养物中获得了197株GABHS分离株。两种培养基在培养48小时后，GABHS的回收率均显著更高。使用ssA平板，在培养24小时以及48小时后，我们检测到的GABHS显著更多。ssA平板在定性和定量方面减少了正常菌群。总之，对于检测GABHS，ssA比羊血琼脂更敏感、更具特异性，而且更易于读数。我们建议培养48小时。
