Schaumburg-Lever G, Metzler G, Kaiserling E
Department of Dermatology, Eberhard-Karls University, Tubingen, Germany.
J Cutan Pathol. 1991 Dec;18(6):432-5. doi: 10.1111/j.1600-0560.1991.tb01380.x.
Three malignant melanomas, two melanoma metastases, two junctional dysplastic nevi, and normal skin were embedded in Lowicryl. Ultrathin sections were incubated with HMB-45 and a gold-labeled anti-mouse antibody. Gold particles indicating the presence of HMB-45 were found in melanosomes Stage 1 and 2 and in the non-melanized portion of melanosomes Stage 3. Melanosomes Stage 4 and melanosome complexes in keratinocytes, as well as in melanophages, were consistently negative. No specific labelling with HMB-45 was seen in eccrine glands of normal skin.
将三个恶性黑色素瘤、两个黑色素瘤转移灶、两个交界性发育异常痣和正常皮肤组织包埋于Lowicryl中。超薄切片与HMB - 45及金标抗小鼠抗体一起孵育。在1期和2期黑素小体以及3期黑素小体的非黑素化部分发现了指示HMB - 45存在的金颗粒。4期黑素小体以及角质形成细胞和噬黑素细胞中的黑素小体复合物始终呈阴性。在正常皮肤的小汗腺中未观察到HMB - 45的特异性标记。
