与番茄水分利用效率相关的限制片段长度多态性。

Restriction fragment length polymorphisms associated with water use efficiency in tomato.

出版信息

Science. 1989 Mar 31;243(4899):1725-8. doi: 10.1126/science.243.4899.1725.

DOI:10.1126/science.243.4899.1725

Abstract

Improvement of crop water use efficiency (WUE) has not been successful because evaluation for this component of drought resistance is unreliable in field-grown plants. The composition of stable isotopes of plant carbon (delta(13)C) was earlier shown to be an excellent indicator of WUE in tomato and other species. It is now reported that delta(13)C can be satisfactorily predicted from three restriction fragment length polymorphisms (RFLPs), which are genetic markers for discrete DNA sequences within the genome. An additive type of gene action was observed for all three RFLP markers, and for one of them there also was a significant nonadditive component. Combining the delta(13)C and RFLP technologies may aid in identifying genes associated with WUE and in studying mechanisms contributing to WUE. These technologies will also be useful for crop improvement.

摘要

提高作物水分利用效率（WUE）一直没有成功，因为在田间生长的植物中，对这种抗旱性组成部分的评估不可靠。植物碳稳定同位素（δ（13）C）的组成较早被证明是番茄和其他物种 WUE 的极好指标。现在据报道，δ（13）C 可以从三个限制片段长度多态性（RFLP）中得到令人满意的预测，RFLP 是基因组内离散 DNA 序列的遗传标记。所有三个 RFLP 标记都观察到了加性类型的基因作用，其中一个标记也存在显著的非加性成分。结合 δ（13）C 和 RFLP 技术可能有助于识别与 WUE 相关的基因，并研究导致 WUE 的机制。这些技术对作物改良也将非常有用。