Paterson A H, Lander E S, Hewitt J D, Peterson S, Lincoln S E, Tanksley S D
Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York 14853.
Nature. 1988 Oct 20;335(6192):721-6. doi: 10.1038/335721a0.
The conflict between the Mendelian theory of particulate inheritance and the observation of continuous variation for most traits in nature was resolved in the early 1900s by the concept that quantitative traits can result from segregation of multiple genes, modified by environmental effects. Although pioneering experiments showed that linkage could occasionally be detected to such quantitative trait loci (QTLs), accurate and systematic mapping of QTLs has not been possible because the inheritance of an entire genome could not be studied with genetic markers. The use of restriction fragment length polymorphisms (RFLPs) has made such investigations possible, at least in principle. Here, we report the first use of a complete RFLP linkage map to resolve quantitative traits into discrete Mendelian factors, in an interspecific back-cross of tomato. Applying new analytical methods, we mapped at least six QTLs controlling fruit mass, four QTLs for the concentration of soluble solids and five QTLs for fruit pH. This approach is broadly applicable to the genetic dissection of quantitative inheritance of physiological, morphological and behavioural traits in any higher plant or animal.
孟德尔颗粒遗传理论与自然界中大多数性状连续变异的观察结果之间的冲突，在20世纪初通过这样一个概念得以解决，即数量性状可能源于多个基因的分离，并受到环境效应的影响。尽管早期的实验表明偶尔可以检测到与此类数量性状基因座（QTL）的连锁，但由于无法用遗传标记研究整个基因组的遗传情况，所以对QTL进行准确而系统的定位一直无法实现。限制性片段长度多态性（RFLP）的应用至少在理论上使此类研究成为可能。在此，我们报告首次在番茄种间回交中使用完整的RFLP连锁图谱将数量性状解析为离散的孟德尔因子。应用新的分析方法，我们定位了至少六个控制果实重量的QTL、四个控制可溶性固形物浓度的QTL和五个控制果实pH值的QTL。这种方法广泛适用于对任何高等植物或动物的生理、形态和行为性状的数量遗传进行遗传剖析。
