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World J Microbiol Biotechnol. 1995 Jul;11(4):438-48. doi: 10.1007/BF00364619.
The exploitation of DNA polymorphisms by an ever-increasing number of molecular marker technologies has begun to have an impact on plant genome research and breeding. Restriction fragment length polymorphisms, micro- and mini-satellites and PCR-based approaches are used to determine inter- and intra-specific genetic diversity and construct molecular maps of crops using specially designed mapping populations. Resistance genes and other agronomically important loci are tagged with tightly linked DNA markers and the genes isolated by magabase DNA technology and cloning into yeast artificial chromosomes (YAC). This review discusses some recent developments and results in this field.
越来越多的分子标记技术正在利用 DNA 多态性，开始对植物基因组研究和育种产生影响。限制性片段长度多态性、微卫星和小卫星以及基于 PCR 的方法被用于确定种间和种内遗传多样性，并使用专门设计的作图群体构建作物的分子图谱。通过与紧密连锁的 DNA 标记标记抗性基因和其他农艺重要基因座，并通过磁珠 DNA 技术分离基因并克隆到酵母人工染色体 (YAC) 中。本文综述了该领域的一些最新进展和成果。
