Palazzo M G, Kalso E, Argiras E, Madgwick R, Sear J
Nuffield Department of Anaesthetics, Oxford, U.K.
Pharmacol Toxicol. 1991 Aug;69(2):107-11. doi: 10.1111/j.1600-0773.1991.tb01281.x.
A double indicator technique has been used in an in situ isolated perfused rabbit lung model to examine the first pass effect of the lung on systemic bupivacaine concentrations. Bupivacaine (0.5 mg/kg) was given in two consecutive boluses to six in situ isolated perfused New Zealand White rabbit lung preparations. The mean recovery (first bolus) of bupivacaine was 62.6% +/- 6.3 (S.E.M.), and 63.7% +/- 10.2 (second bolus), suggesting bupivacaine accumulation in the lung. The average mean transit time for bupivacaine was 280.5% +/- 24.1 and 264.8% +/- 36.7 longer than ICG (Indocyanine Green) following the first and second boluses respectively (P less than 0.01). There were no differences in the first pass effect of the lung between the first and second boluses of bupivacaine. The profiles of the bupivacaine concentrations suggest that uptake is followed by accumulation and later back diffusion. This has implications for conditions that decrease the uptake and therefore increase the risk of systemic toxicity.
一种双指示剂技术已用于原位分离灌注兔肺模型，以研究肺对全身布比卡因浓度的首过效应。将布比卡因（0.5mg/kg）分两次连续推注给予六个原位分离灌注的新西兰白兔肺标本。布比卡因的平均回收率（首次推注）为62.6%±6.3（标准误），第二次推注为63.7%±10.2，提示布比卡因在肺中蓄积。首次和第二次推注后，布比卡因的平均通过时间分别比吲哚菁绿（ICG）长280.5%±24.1和264.8%±36.7（P<0.01）。布比卡因首次和第二次推注之间肺的首过效应没有差异。布比卡因浓度曲线表明，摄取后接着是蓄积，随后是反向扩散。这对降低摄取从而增加全身毒性风险的情况具有影响。
