Takahashi H, Ikarashi T, Kanazawa K, Kominami R, Tanaka K
Department of Obstetrics and Gynecology, Shibata Prefectural Hospital, Niigata, Japan.
Placenta. 1991 Sep-Oct;12(5):487-93. doi: 10.1016/0143-4004(91)90025-b.
Ten cases of hydatidiform mole (HM) were analysed by the DNA fingerprint method. DNA samples were prepared from HM tissue of HM and maternal and paternal bloods, followed by digestion with HaeIII restriction endonuclease, applied to agarose gel, and then transferred to a nitrocellulose filter. The filter was hybridized using mini-satellite DNA as a probe. A case of partial HM diagnosed by histological findings was revealed to be complete HM using DNA fingerprinting. The case was suggested to be a twin pregnancy with a cHM and an abortion, because the DNA fingerprint of hydropic tissue showed a paternal pattern and that of the non-hydropic tissue did not show such a pattern. DNA fingerprinting was considered to be useful in distinguishing complete HM from partial HM.
采用DNA指纹法分析了10例葡萄胎（HM）。从HM组织、母体血液和父体血液中制备DNA样本，然后用HaeIII限制性内切酶消化，应用于琼脂糖凝胶，再转移到硝酸纤维素滤膜上。用小卫星DNA作为探针进行滤膜杂交。通过组织学检查诊断为部分性HM的1例病例，经DNA指纹分析显示为完全性HM。该病例被认为是双胎妊娠，其中一个为完全性葡萄胎（cHM），另一个为流产，因为水肿组织的DNA指纹显示为父系模式，而非水肿组织则未显示这种模式。DNA指纹分析被认为有助于区分完全性HM和部分性HM。
