Neulen J, Williams R F, Schaefer W, Flecken U, Hodgen G D
Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23510.
Prostaglandins. 1991 Aug;42(2):121-5. doi: 10.1016/0090-6980(91)90071-m.
Preimplantation embryos of many species are known to synthesize prostaglandins. These tissue hormones are believed to influence embryonic metabolism, as well as embryo-maternal interaction during implantation although their putative role(s) remains obscure. Here, prostaglandin production by blastocysts from cynomolgus monkeys (Macaca fascicularis) was examined qualitatively during in vitro culture. Tritium labelled arachidonic acid was metabolized to 6 keto-prostaglandin F1 alpha, 2,3-dinor-prostaglandin F1 alpha and thromboxane B2, as characterized by HPLC separation. Also, 6-keto-prostaglandin F1 alpha, and thromboxane B2 as characterized by HPLC separation. Also, 6-keto-prostaglandin F1 alpha and thromboxane B2 were identified by specific RIA's. Our data suggest that the main arachidonic acid metabolites produced by blastocysts of cynomolgus monkeys are prostacyclin and thromboxane.
已知许多物种的植入前胚胎会合成前列腺素。这些组织激素被认为会影响胚胎代谢以及植入过程中的胚胎与母体相互作用，尽管它们的假定作用仍不清楚。在此，对食蟹猴（猕猴）囊胚在体外培养期间的前列腺素产生进行了定性检测。经高效液相色谱分离鉴定，氚标记的花生四烯酸代谢为6-酮-前列腺素F1α、2,3-二去甲-前列腺素F1α和血栓素B2。此外，经高效液相色谱分离鉴定出了6-酮-前列腺素F1α和血栓素B2。同时，通过特异性放射免疫分析法鉴定出了6-酮-前列腺素F1α和血栓素B2。我们的数据表明，食蟹猴囊胚产生的主要花生四烯酸代谢产物是前列环素和血栓素。
