Woo Sang Uk, Bae Jeoung Won, Yang Jung-Hyun, Kim Jung Han, Nam Seok Jin, Shin Young Kee
Department of Surgery, Korea University College of Medicine, Seoul, Korea.
Ann Surg Oncol. 2007 Nov;14(11):3268-73. doi: 10.1245/s10434-007-9532-3. Epub 2007 Aug 29.
In breast carcinoma, identification of tumor cells in the sentinel lymph nodes is a predictor of the tumor's metastatic potential. Sentinel lymph node may be targeted not only by tumor cell metastasis but also by cytokines from the emergence of antitumor immune responses.
Between February 2003 and February 2004, the investigator evaluated 38 cases that underwent sentinel lymph node biopsy at the Samsung Medical Center. Eighty paraffin-embedded sections, 49 sentinel, and 31 nonsentinel lymph node, from breast carcinoma without lymphatic metastases were analyzed by real-time polymerase chain reaction to evaluate the cytokine profile (interferon-gamma, interleukin-2, interleukin-10 and interleukin-12) for the T cell response.
A higher expression of interleukin-10 was observed in sentinel lymph node than in nonsentinel lymph node (P = 0.03). The expressions of interferon-gamma, interleukin-2, and interleukin-12 were similar between sentinel and nonsentinel lymph node.
Theses results indicate that T cell response was downregulated by interleukin-10 overexpression in sentinel lymph node with breast cancer.
在乳腺癌中，前哨淋巴结中肿瘤细胞的识别是肿瘤转移潜能的一个预测指标。前哨淋巴结不仅可能受到肿瘤细胞转移的靶向作用，还可能受到抗肿瘤免疫反应产生的细胞因子的靶向作用。
在2003年2月至2004年2月期间，研究者评估了三星医疗中心38例接受前哨淋巴结活检的病例。对80个石蜡包埋切片，49个前哨淋巴结和31个非前哨淋巴结进行分析，这些样本均来自无淋巴转移的乳腺癌患者，通过实时聚合酶链反应评估T细胞反应的细胞因子谱（干扰素-γ、白细胞介素-2、白细胞介素-10和白细胞介素-12）。
在前哨淋巴结中观察到白细胞介素-10的表达高于非前哨淋巴结（P = 0.03）。前哨淋巴结和非前哨淋巴结中干扰素-γ、白细胞介素-2和白细胞介素-12的表达相似。
这些结果表明，在乳腺癌患者的前哨淋巴结中，白细胞介素-10的过表达下调了T细胞反应。
