Traitcheva Nelly, Jenke-Kodama Holger, He Jing, Dittmann Elke, Hertweck Christian
Department of Biomolecular Chemistry, Leibniz-Institute for Natural Product Research and Infection Biology (HKI), Beutenbergstrasse 11a, 07745 Jena, Germany.
Chembiochem. 2007 Oct 15;8(15):1841-9. doi: 10.1002/cbic.200700309.
Aureothin and neoaureothin (spectinabilin) represent rare nitroaryl-substituted polyketide metabolites from Streptomyces thioluteus and Streptomyces orinoci, respectively, which only differ in the lengths of the polyene backbones. Cloning and sequencing of the 39 kb neoaureothin (nor) biosynthesis gene cluster and its comparison with the aureothin (aur) pathway genes revealed that both polyketide synthase (PKS) assembly lines are remarkably similar. In both cases the module architecture breaks with the principle of colinearity, as individual PKS modules are used in an iterative fashion. Parsimony and neighbour-joining phylogenetic studies provided insights into the evolutionary process that led to the programming of these unusual type I PKS systems and to prediction of which modules act iteratively. The iterative function of the first module in the neoaureothin pathway, NorA, was confirmed by a successful cross-complementation.
金硫素和新金硫素（spectinabilin）分别是来自硫黄链霉菌和奥里诺科链霉菌的罕见硝基芳基取代聚酮化合物代谢产物，它们仅在多烯主链长度上有所不同。对39 kb新金硫素（nor）生物合成基因簇的克隆和测序，并将其与金硫素（aur）途径基因进行比较，结果表明这两条聚酮合酶（PKS）装配线非常相似。在这两种情况下，模块结构都不符合共线性原则，因为单个PKS模块是以迭代方式使用的。简约法和邻接法系统发育研究为导致这些不寻常的I型PKS系统编程的进化过程以及预测哪些模块以迭代方式起作用提供了见解。新金硫素途径中第一个模块NorA的迭代功能通过成功的交叉互补得到了证实。
